Capillary Electrophoresis: Theory and PracticePaul D. Grossman, Joel C. Colburn Academic Press, 2 thg 12, 2012 - 352 trang This book is designed to be a practical guide, used by wide audience, including those new to CE, those more experienced, routine users, those interested in technology development, and those involved with applications research. References have been emphasized to allow the reader to explore the detailed specifics and theoretical foundations. This book draws together the rapidly evolving, diverse, and multidisciplinary subject of capillary electrophoresis (CE). It is designed as a practical guide to be used by a wide audience, including those new to CE as well as more experienced users. This volume presents the capabilities, limitations, potentials, and future challenges facing each area of CE. Key aspects of this technique, such as high resolution capability, full automation, high speed separations, quantification of nanoliter sample volumes, and simultaneous multiple detection capabilities are presented in a concise and logical fashion. This book is designed to help you make the most of your CE separations, and includes comprehensive information on:Electroosmosis, separation efficiency, and Joule heatingDetection methodsIn-depth discussion of the separation principles and capabilities of the major modes of CESieving gel electrophoresisIsoelectric focusingFree solution CEMicellar electrokinetic capillary chromatographyEntangled polymer matrix-based separationDetailed treatment of the application of CE to a wide range of molecules, supplemented with extensive "hands-on" illustrations |
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amino acids ampholytes Anal analysis analyte anions applied Balchunas capillary diameter capillary electrophoresis capillary length capillary wall cationic charge Chem chiral Chromatogr chromatography cm to detector coefficient concentration Confirm convection defined derivatization detection limits difficult diffusion effect efficiency elec electrical field strength electrode electrokinetic electroosmotic flow electropherogram electrophoresis capillary electrophoretic mobility elution range fiber Figure first flow fused-silica gel electrophoresis gel-filled capillary Grossman Hjertén HPCE HPLC hydrophobic i.d. capillary increase influence injection interactions ionic isoelectric focusing Jorgenson Joule heating Karger laser linear MECC method micellar micelle migration mobile phase molecular molecules myoglobin neutral Nishi oligonucleotides optical organic modifier parameters peak peptides phosphate polymer profile proteins quantitative Reproduced with permission resolution result sample sensitivity Sepaniak separation significant sodium solution solvent specific sufficient surfactants technique temperature Terabe theoretical plates tion tricine tube UV-absorbance V/cm velocity voltage volume Wallingford wavelength zone